ABCG1 (ATP-binding Cassette (ABC) Transporter 1)

The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells. In addition, increasing numbers of ABC proteins have recently been identified. The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules. ABCG proteins were able to transport substances across cellular membranes and against concentration gradient they require an input of energy, which requires the hydrolysis of ATP, directly or indirectly.\nABCG1 a 678aa protein in human (chr 21q22.3), 666aa in mouse, predominantly expressed in many tissues, in the intracellular compartments mainly associated with endoplasmic reticulum and Golgi complex. It shows 26% sequence homology with ABCG2 protein. ABCG1 protein is involved in macrophage lipid homeostasis, an active member of macrophage lipid export complex.\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1-10ug/ml using ECL.\nELISA: 0.5-1ug/ml Use A0004-04M1 to coat ELISA plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0004-04M

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Matthew A. Kennedy et al (2001) JBC, Vol. 276, No: 42, I39438-39447; 2. Schmitz, G et al (2001), J. Lipid Res. 42 (10), 1513-1520; 3. Lorkowski, S. et al (2001) Biochem. Biophys. Res Commun. 280 (1), 121-131; 4. Venkateswaran, A et al (2000) JBC. 275 (19), 14700-14707.

Additional Info

Recognizes mouse ABC G1. Species sequence homology: rat-100%, human-92%.