ACAD8 (Acyl-Coenzyme A Dehydrogenase 8, Mitochondrial) Recombinant, Human

Acyl-coenzyme A (CoA) dehydrogenases (ACADs) are a family of mitochondrial enzymes that catalyze the first dehydrogenation step in the bets-oxidation of fatty acyl-CoA derivatives. Several human ACADs exist and all ACADs catalyze the same initial dehydrogenation of the substrate at the beta-carbon atom and require electron transfer flavoprotein as an alectron acceptor. The predicted 415-amino acid ACAD8 protein contains many of the residues conserved in most other ACADs, including an active site glutamic acid residue and residues important for tetramer formation.\n\nDescription: \nRecombinant Human ACAD-8 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-415 and having a total molecular mass of 47.7kD. Recombinant Human ACAD8 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.\n\nApplications: \n

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SPECIFICATIONS

Catalog Number

A0195-03

Size

2ug

Form

Supplied as a liquid in 10mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% sodium azide, 10mM DTT.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Sudden death in a young woman from medium chain acyl-coenzyme A dehydrogenase (MCAD) deficiency. J Emerg Med 2006 Apr;30(3):291-4 2. Suppression of estrogen-related receptor alpha and medium-chain acyl-coenzyme A dehydrogenase in the acute-phase response. J Lipid Res 2005 Oct;46(10):2282-8 3. L-carnitine and exercise tolerance in medium-chain acyl-coenzyme A dehydrogenase (MCAD) deficiency: a pilot study. J Inherit Metab Dis 2005;28(2):141-52 4. Functional interference between estrogen-related receptor alpha and peroxisome proliferator-activated receptor alpha/9-cis-retinoic acid receptor alpha heterodimer complex in the nuclear receptor response element-1 of the medium chain acyl-coenzyme A dehydrogenase gene. J Mol Endocrinol 2003 Aug;31(1):47-60 5. Very-long-chain acyl-coenzyme a dehydrogenase deficiency in mice. Circ Res 2003 Sep 5;93(5):448-55 6. Comparison of ligand polarization and enzyme activation in medium- and short-chain acyl-coenzyme A dehydrogenase-novel analog complexes. Arch Biochem Biophys 2003 Jan 15;409(2):251-61