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ACADL, Recombinant, Human, aa31-430, His-tag (Long-chain Specific Acyl-CoA Dehydrogenase, Mitochondrial, LCAD)

Cat no: A0193-03

ACADL, Recombinant, Human, aa31-430, His-tag (Long-chain Specific Acyl-CoA Dehydrogenase, Mitochondrial, LCAD)

ACADL belongs to the acyl-CoA dehydrogenase family, existing as a homotetramer. It is involved in the catabolism of fatty acids and aa and they provide a major source of energy for the heart and skeletal muscle. Defect in the gene encoding ACADL leads to non-ketotic hypoglycemia and hypotonia, muscle weakness.\n\nSource:\nRecombinant corresponding to aa31-430 from human ACADL, fused to His-tag at N-terminal expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGGEERLETP SAKKLTDIGI RRIFSPEHDI FRKSVRKFFQ EEVIPHHSEW EKAGEVSREV WEKAGKQGLL GVNIAEHLGG IGGDLYSAAI VWEEQAYSNC SGPGFSIHSG IVMSYITNHG SEEQIKHFIP QMTAGKCIGA IAMTEPGAGS DLQGIKTNAK KDGSDWILNG SKVFISNGSL SDVVIVVAVT NHEAPSPAHG ISLFLVENGM KGFIKGRKLH KMGLKAQDTA ELFFEDIRLP ASALLGEENK GFYYIMKELP QERLLIADVA ISASEFMFEE TRNYVKQRKA FGKTVAHLQT VQHKLAELKT HICVTRAFVD NCLQLHEAKR LDSATACMAK YWASELQNSV AYDCVQLHGG WGYMWEYPIA KAYVDARVQP IYGGTNEIMK ELIAREIVFD K\n\nMolecular Weight:\n~46.7kD (MALDI-TOF)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0193-03

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol, 1mM DTT, 0.15M sodium chloride.

Purity

~85% (SDS-PAGE)

References

1. Maher AC., et al. (2010) Mol Genet Metab. 100(2):163-7. 2. Wanders RJ., et al. (1998) Biochim Biophys Acta. 1393(1):35-40.

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