Actin, alpha, Sarcomeric

Actin is a highly conserved protein which participates in a wide variety of cellular functions in eucaryotes including muscle contraction, amoeboid movement, cytokinesis, and mitotic division. Humans have six actin genes; two striated muscle types or sarcomeric (alpha-skeletal and alpha-cardiac), two smooth muscle types and two cytoskeletal forms (beta and gamma-types). The cytoplasmic beta and gamma actins are coexpressed in mammalian non-muscle cells, while the two smooth muscle actins are coexpressed only in smooth muscle with their relative amounts varying in different smooth muscle types. Similarly the sarcomeric actins, alpha-skeletal actin and alpha-cardiac actin are expressed in adult skeletal muscle and adult heart muscle respectively. Sarcomeric actins are major components of the thin filaments of striated muscle and it is the regulated interactions of actins by tropomyosin and the troponin complex with myosins of the thick filament which are responsible for contraction and generation of force. The two sarcomeric actin isoforms are encoded by different genes and are highly conserved. These two isoactins differ markedly in the 5' and 3' untranslated regions of the RNA and by fouraa at the protein level. The amount of the isoforms and the respective mRNA present varies with species, muscle type, development and aging.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSkeletal muscle\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0759-03C

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum, Rat, Bov, GP, Rabbit, Sheep

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgM

References

1. Babai et al. Virchow Arch 55: 263, 1988. 2. Schurch et al. Amer J Pathol 128:91, 1987. 3. Skalli et al. Am Amer J Pathol 130: 515, 1988. 4. Rubensteinet al. Bioessays 12: 309, 1990. 5. Boheler et al. J Clin Invest 88: 323, 1991.

Additional Info

Recognizes rabbit Actin Sarcomeric. This antibody is specific to alpha-skeletal and alpha-cardiac muscle actins. Does not reacts with smooth muscle tissue. Species Crossreactivity: bovine, carp, frog, guinea pig, human, rat, sheep, snake.