Actin, alpha, Smooth Muscle (BSA, Glycerol & Azide Free)

Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.\n\nApplications: \nSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution: \nImmunohistochemistry (Frozen & formalin/paraffin): 0.5-1ug/ml for 30min at RT. No special pretreatment is required for the Immunohistochemical staining of formalin/paraffin tissues. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSmooth muscle or leiomyosarcoma\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0760-26X

Size

100ug

Applications

IF, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, Rabbit

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives added. No stabilizing proteins added. Glycerol free. Also available with BSA and azide. See cat# A0760-26.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. Bochaton-Piallat ML; Gabbiani F; Gabbiani G. Heterogeneity of rat aortic smooth muscle cell replication during development: correlation with replicative activity after experimental endothelial denudation in adults. Journal of Submicroscopic Cytology and Pathology, 1994, 26(1):1-8. 2. Cremona O; Savoia P; Marchisio PC; Gabbiani G; Chaponnier C. The alpha 6 and beta 4 integrin subunits are expressed by smooth muscle cells of human small vessels: a new localization in mesenchymal cells. Journal of Histochemistry and Cytochemistry, 1994, 42(9):1221-8. 3. Estes JM; Vande Berg JS; Adzick NS; MacGillivray TE; Desmouliere A; Gabbiani G. Phenotypic and functional features of myofibroblasts in sheep fetal wounds. Differentiation, 1994, 56(3):173-81. 4. Orlandi A; Ehrlich HP; Ropraz P; Spagnoli LG; Gabbiani G. Rat aortic smooth muscle cells isolated from different layers and at different times after endothelial denudation show distinct biological features in vitro. Arteriosclerosis and Thrombosis, 1994, 14(6):982-9.

Additional Info

Recognizes the alpha-smooth muscle isoform of actin. Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. Reacts with tumors arising from smooth muscles and myoepithelial cells. Does not crossreact with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial). Species Crossreactivity: Human, baboon, bovine, rabbit, mouse, rat and chicken.