The Human Activated Protein C gla domainless was prepared from purified Human APCusing Cathepsin G. The Cathepsin G is removed using affinity chromatography afterdigestion. The above protein was purified from Human plasma that was tested and foundnegative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV,Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors arescreened for CJD (Creutzfeldt-Jakob Disease)