Mouse IL-1beta ELISA kit is designed for detection of Mouse IL-1beta in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures IL-1beta in less than 5 hours. A murine monoclonal antibody specific for IL-1beta has been pre-coated onto a microplate. IL-1beta in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for IL-1beta, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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