Activating Transcription Factor 2, Recombinant, Human (ATF2, CREB P1)

Recombinant Human ATF-2 produced in E.Coli is a non-glycosylated, polypeptide chain encoding 19-96 amino acids and having a molecular mass of 57kD. The recombinant ATF-2 is manufactured as a maltose binding protein (MBP) fusion protein with an amino terminal polyhistidine tag. Human ATF-2 is purified by proprietary chromatographic techniques.\n\nUnit Definition: \nRecombinant human ATF2 is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with immunoprecipitated JNK from stimulated cells. This phosphorylation can be monitored by Western blot analysis using phosphorylation site specific antibody directed to ATF2 [pT71], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application. Please note: Kinase activity may vary depending on the substrate and reaction conditions.\n\nStorage: \nStore at 4 degrees C if entire vial will be used within 1-2 weeks. Store, frozen at -20 degrees C for longer periods of time. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

A0855-42J

Size

2ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 90% (SDS-PAGE)

References

1. Expression of transcription factors c-Fos, c-Jun, CREB-1 and ATF-2, and caspase-3 in relation with abnormal tau deposits in Pick's disease. Acta Neuropathol (Berl) 2006 Apr;111(4):341-50 2. Prolonged activation of cAMP-response element-binding protein and ATF-2 needed for nicotine-triggered elevation of tyrosine hydroxylase gene transcription in PC12 cells. J Biol Chem 2006 Apr 14;281(15):10188-95 3. Cyclin D1 activation through ATF-2 in Reg-induced pancreatic beta-cell regeneration. FEBS Lett 2006 Jan 23;580(2):585-91 4. The bZip proteins CES-2 and ATF-2 alter the timing of transcription for a cell-specific target gene in C. elegans. Dev Biol 2006 Jan 15;289(2):456-65 5. TGF-beta1 induces cardiac hypertrophic responses via PKC-dependent ATF-2 activation. J Mol Cell Cardiol 2005 Oct;39(4):627-36 6. Activation of cyclin D1 by estradiol and spermine in MCF-7 breast cancer cells: a mechanism involving the p38 MAP kinase and phosphorylation of ATF-2. Oncol Res 2005;15(3):113-28