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Activating Transcription Factor 3 , Recombinant, Human, aa1-181 (ATF3, cAMP-dependent Transcription Factor ATF-3, Cyclic AMP-dependent Transcription Factor ATF-3)

Cat no: A0855-58J

Activating Transcription Factor 3 , Recombinant, Human, aa1-181 (ATF3, cAMP-dependent Transcription Factor ATF-3, Cyclic AMP-dependent Transcription Factor ATF-3)

ATF3 (activating transcription factor 3) is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription, it is presumed, by sequestering inhibitory co-factors away from the promoter. \n\nSource:\nRecombinant correspnding to aa1-181 of human ATF3, expressed in E.coli.\n\nAA Sequence:\nMMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK LESVNAELKA QIEELKNEKQ HLIYMLNLHR PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S\n\nMolecular Weight: \n~20.6kD (181aa)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0855-58J

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH8.0, 2M Urea, 20% glycerol.

Purity

~90% (SDS-PAGE)

References

Lv D., et al. (2011) IUBMB Life. 63(1):62-9. Chen BP., et al. (1994) J Biol Chem 269(22):15819

Read more on Supplier website

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info
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