ADAM23 is a 100kD member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum and serves as a counter-receptor for alphav, beta3 integrin. The human ADAM23 proprecursor is a 773aa type I transmembrane (TM) protein. It contains a 227aa
cleavable proregion (aa60-286) and a 506aa extracellular domain (ECD) (aa287-792) that is part of a 70kD mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa 299-494), an integrin-binding disintegrin region (aa511-585) and a Cys-rich domain (aa589-611). Two splice variants exist. One shows an in-frame 46aa substitution for aa787-832 that generates a soluble form, while a second shows an in-frame 31aa TM substitution for aa787-817. Over aa1-585, human ADAM23 is 92% aa identical to mouse ADAM23.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.1-0.2ug/ml. The detection limit is ~10ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS (20mM Trizma base, 150mM sodium chloride), pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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