ADAM 23 (A Disintegrin And Metalloproteinase 23) (Biotin)

ADAM23 is a 100kD member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum and serves as a counter-receptor for alphav, beta3 integrin. The human ADAM23 proprecursor is a 773aa type I transmembrane (TM) protein. It contains a 227aa \ncleavable proregion (aa60-286) and a 506aa extracellular domain (ECD) (aa287-792) that is part of a 70kD mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa 299-494), an integrin-binding disintegrin region (aa511-585) and a Cys-rich domain (aa589-611). Two splice variants exist. One shows an in-frame 46aa substitution for aa787-832 that generates a soluble form, while a second shows an in-frame 31aa TM substitution for aa787-817. Over aa1-585, human ADAM23 is 92% aa identical to mouse ADAM23. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.2ug/ml. The detection limit is ~10ng/lane under non-reducing and reducing conditions.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS (20mM Trizma base, 150mM sodium chloride), pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0859-41C

Size

50ug

Applications

WB

Hosts

Sheep

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS (20mM Trizma base, 150mM sodium chloride), pH 7.3, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human ADAM23. Shows <5% crossreactivity with recombinant human ADAM8, -9 and -22.