Agarase, beta (b-Agarase)

b-Agarase is used to purify nucleic acids and DNA fragments from low melting agarose gels by hydrolyzing the polysaccharide core of 1,3-b,D-galactopyranose into neoagarobiose oligosaccharides.\n\nUnit Definition:\nOne unit of b-Agarase is defined as the amount of enzyme necessary to produce 1ug of reducing sugar as D-Galactose from agar at pH 6.0 at 40 degrees C per minute.\n\nActivity:\n~1000-3000U/mg\n\nApplication:\nQuantitative recovery of DNA or RNA from low melting point agarose gels. The recovered nucleic acids can be subjected to many further manipulations such as restriction endonuclease digestion, ligation, labeling, sequencing, amplification, etc.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0965

Size

500U

Form

Supplied as a liquid in phosphate buffer salts, BSA.

References

1. Morrice, L.M., et al., Eur. J. Biochem. 135: 553-558 (1983). 2. Yaphe, W., Can. J. Microb. 3: 987-993 (1957). 3. Duckworth, M., Turvey, J.R., Biochem. J. 113:687-692 (1969). 4. Sambrook, J., Russell, D.W., Molecular Cloning: A laboratory Manual, third ed., CSHL Press (2001). 5. Young, K.S., et al., Enzymatic cleavage of the b