Agarase, beta (b-Agarase)

Cat no: A0965

Supplier: United States Biological

0 reviews | Write a Review Pencil

Read More on Supplier Website

Description
Additional Information

b-Agarase is used to purify nucleic acids and DNA fragments from low melting agarose gels by hydrolyzing the polysaccharide core of 1,3-b,D-galactopyranose into neoagarobiose oligosaccharides. Unit Definition: One unit of b-Agarase is defined as the amount of enzyme necessary to produce 1ug of reducing sugar as D-Galactose from agar at pH 6.0 at 40 degrees C per minute. Activity: ~1000-3000U/mg Application: Quantitative recovery of DNA or RNA from low melting point agarose gels. The recovered nucleic acids can be subjected to many further manipulations such as restriction endonuclease digestion, ligation, labeling, sequencing, amplification, etc. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

See Full Product Listings >

Catalogue number:	A0965
Size:	500U
Form:	Supplied as a liquid in phosphate buffer salts, BSA.
References:	1. Morrice, L.M., et al., Eur. J. Biochem. 135: 553-558 (1983). 2. Yaphe, W., Can. J. Microb. 3: 987-993 (1957). 3. Duckworth, M., Turvey, J.R., Biochem. J. 113:687-692 (1969). 4. Sambrook, J., Russell, D.W., Molecular Cloning: A laboratory Manual, third ed., CSHL Press (2001). 5. Young, K.S., et al., Enzymatic cleavage of the b

Agarase, beta (b-Agarase)

Cat no: A0965

Get Quote

About Biosave

Product Categories

Information


Get a Quote Direct from United States Biological




By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*