Agouti Protein

Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132aa in human) is normally expressed in skin. Its ubiquitous expression, such as in rare allele lethal yellow, causes yellow coat., linear growth, immune defects and obesity. Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1 and possibly MC2R and MC4R).\n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 (ECL).\nELISA: 1:10,000-1:100,000 using 50-100ng control peptide (A1059-58A) per well.\nOptimal dilutions to be determined by researcher.\n\nControl Peptide:\nA1059-58A: Agouti (Control Peptide)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1059-59B

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Miller, M.W., et al., Genes Develop. 7: 454 (1993). 2. Bultman. S.J., et al., Cell 71: 1195 (1992). 4. Kwon, H.Y., et al., PNAS 91: 97601 (1994). 5. Wilson, B.D., Human Mol. Genet. 4: 223 (1995).

Additional Info

Recognizes mouse Agouti. No homology is seen with AGRP, tubby, tub, TULP1 and TULP2.