Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA (capture): 1ug/ml using 100ul/well.
Western Blot: 1-2ug/ml Detection limit for recombinant human AgRP and recombinant mouse AgRP is ~5ng/lane under non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Recommended Pair:
Capture: A1059-62L
Detection: A1059-62M
Hybridoma:
Myeloma with B cells from Balb/c mice.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.