AKT3, ID (RAC-gamma Serine/Threonine-protein Kinase, RAC-PK-gamma, Protein Kinase Akt-3, Protein Kinase B, gamma, PKB gamma, STK-2, PKBG)

Supplier:

United States Biological

Cat no: A1125-30S

AKT3, ID (RAC-gamma Serine/Threonine-protein Kinase, RAC-PK-gamma, Protein Kinase Akt-3, Protein Kinase B, gamma, PKB gamma, STK-2, PKBG)

The serine-threonine kinase Akt, also known as protein kinase B (PKB), is an important effector for phosphatidylinositol 3-kinase signaling initiated by numerous growth factors and hormones. Akt2/PKB beta, one of three known mammalian isoforms of Akt/PKB, has been demonstrated recently to be required for at least some of the metabolic actions. Over expression of exogenous Akt enhances cell survival by dramatically reducing apoptosis and transfection of dominant negative mutant kinase constructs (kinase-dead) inhibits survival of neurons promoted by IGF-1. Akt2 is also expressed in human carcinoma cell lines and primary tumors.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1125-30S

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Cho, H. et al. Science 292, 1728 (2001). 2. Vanhaesebroeck, B. et al. Biochem J 346, 571 (2000). 3. Brodbeck, D. et al. J Biol Chem 274, 9133 (1999). 4. Masure, S. et al. Eur J Biochem 265, 353 (1999). 5. Nakatani, K. et al. J Biol Chem 274, 21528 (1999). 6. Jones, P. et al. Proc Natl Acad Sci USA 88, 4171 (1991).

Additional Info

Recognizes AKT3. Species Crossreactivity: human and mouse.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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