Alanine Transaminase, Recombinant, Human (ALT, Alanine Aminotransferase, Serum Glutamic Pyruvic Transaminase, SGPT)

Alanine aminotransferase (ALT, GPT, Gutamate pyruvate transaminanse), catalyzes the transamination reaction. ALT requires pyridoxal-5-phosphate (PLP) as prosthetic group. ALT was previously known as serum glutamic pyruvic transaminase (SGPT). ALT is a cytoplasmic enzyme that catalyzes the transamination of alpha-ketoglutarate and L-alanine, forming glutamate and pyruvate. ALT is found in liver, kidney, brain and heart , as two isoforms: cytosolic ALT and mitochondrial ALT. These enzymes function in cellular nitrogen metabolism and also liver gluconeogenesis. The highest activities of ALT are found in hepatocytes and striated (skeletal and cardiac) muscle cells. Therefore, increased serum ALT activity can accompany hepatocellular injury or necrosis of striated muscle. With cell injury or death, ALT (a "leakage" enzyme) escapes from the cytosol. Determination of ALT activity is a relatively sensitive indicator of hepatic damage in certain animal species and can help determine whether further diagnostic tests.\n\nRecombinant ALT is a single, non-glycosylated, polypeptide chain containing 495 amino acids and having a molecular mass of 54,479 Dalton and is produced as fully active cytosolic soluble protein in E. coli by expressing the gene encoding cytosolic human Alanine Aminotransferase. \n\nEC. 2.6.1.2\n\nApplications: \nSuitable for use as a Calibrator/Control for ALT activity assay. Standard antigen for Western Blot or ELISA. Marker for Hepatic cell research. Other applications not tested.\n\nRecommended Dilutions: Optimal dilutions to be determined by the researcher.\n\nBiological Activity: ~1000u/ml\n\nSpecific Activity:\n~900U/mg protein\n\nGene: Name: GPT\n\nSynonyms: AAT1, GPT1\n\nProtein synonyms/aliases:\nAlanine aminotransferase 1 (EC 2.6.1.2) (ALT1)\n(Glutamic--pyruvictransaminase 1) (GPT 1)\n(Glutamic--alanine transaminase 1). \n\nProtein Family:\nBelongs to the class-I pyridoxal-phosphate-dependent \n\nProtein Domains:\nIPR004839 Aminotransferase, class I and II\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1210-30B

Size

5U

Form

Supplied as a liquid in 40mM Na acetate buffer, 1mM DTT, 1mM EDTA, 5mM 2-oxoglutarate, and 0.1mM pyridoxal 5

Purity

(same/more than)95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Rossi G, Recenti Prog Med 2004 Nov;95(11):509-11 2. Sattar N, Scherbakova O, O'Reilly DS, Packard CJ, Diabetes 2004 Nov;53(11):2855-60 3. Tanaka H, Tsukuma H, Oshima A, Int J Cancer 2004 Dec 20;112(6):1075-80 4. Fonquernie L, Serfaty L, Wendum D, HIV Med 2004 Sep;5(5):385-90 5. Terabe K, Sugiyama K, Mizutani F, Tohoku J Exp Med 2004 Aug;203(4):339-44 6. Fernandez-Real JM, Lopez-Bermejo A, Vendrell J, Clin Chem 2004 Aug;50(8):1456-