Albumin, BCP (Bromcresol Purple) Assay Kit, BioAssay(TM)

Albumin is the most abundant plasma protein in human. It accounts for about 60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure, binding of key substances such as long-chain fatty acids, bile acids, bilirubin, haematin, calcium, magnesium. It has anti-oxidant and anticoagulant effects, and also acts as a carrier for nutritional factors and drugs, as an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition and protein-losing enteropathies. High levels are associated with dehydration.\n\nSimple, direct and automation-ready procedures for measuring albumin concentration in biological samples are becoming popular in Research and Drug Discovery. BCP albumin assay kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol purple that forms a colored complex specifically with albumin. The intensity of the color, measured at 610nm, is directly proportional to the albumin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.\n\nKey Features:\nSensitive and accurate. Use as little as 20ul samples. Detection range 0.3 g/dL (45uM) to 5 g/dL (750uM) albumin in 96-well plate assay.\nSimple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day.\nImproved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.\nLow interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein.\n\nApplications:\nDirect Assays: albumin in serum, urine, biological preparations.\nDrug Discovery/Pharmacology: effects of drugs on albumin metabolism.\n\nKit Contents:\n1. Reagent, 1x50ml \n2. Albumin standard (5g/dL), 1x2ml \n\nStorage and Stability:\nThe kit is shipped at room temperature. Store Reagent at 4 degrees C and Albumin standard at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1309-52

Size

1Kit

References

1. Maier SM et al (2007) Proteinuria of Nonautoimmune Origin in Wild-type FVB/NJ Mice. Comp Med. 57(3):255-66.\n2. Sharifuzzaman SM and Austin B (2009). Kocuria SM1 controls vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum). J Appl Microbiol. 108(6): 2162