Albumin, Human Serum (HSA)

Human serum albumin is a single chain polypeptide which composes about 60% of the plasma proteins. In normal serum approximately 10% of the albumin is modified by non-enzymatic glycosylation which occurs primarily on lysine residue number 525. The amount of glycosylated albumin in serum is markedly elevated in diabetes and thus its determination may help in the monitoring of the disease. Studies also show that glycosylation of the protein alters its properties and this modification causes it to be ingested by endothelial cells in preference to unmodified albumin. Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: Indirect\nWestern Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1903-80: IgY, H&L, X-Adsorbed, 12nm (Gold) Pab Dk xCh\nI1903-80A: IgY, H&L, X-Adsorbed, 18nm (Gold) Pab Dk xCh\nI1903-80B: IgY, H&L, X-Adsorbed, 6nm (Gold) Pab Dk xCh\nI1906: IgY Pab Rb xCh\nI1906-01: IgY, Fc (HRP) Pab Gt xCh\nI1906-10: IgY (HRP) Pab Rb xCh\nI1906-12: IgY (FITC) Pab Rb xCh\nI1906-14: IgY (AP) Pab Rb xCh\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1274-88

Size

50ug

Applications

ELISA, WB

Hosts

Ch/Bird

Reactivities

Hum, Mouse, Rat, Bov, Can, Prc

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgY

References

1. Dugaiczyk, A., Law, S.W., Dennison, O.E.: Nucleotide sequence and the encoded amino acids of human serum albumin mRNA. PNAS USA 79(1): 71-75 (1982).

Additional Info

Recognizes human serum albumin. Species Crossreactivity: Mouse, rat, bovine, porcine and canine.