Albumin, Human Serum Recombinant (HSA)

Serum albumin is the most abundant blood plasma protein and is produced in the liver and forms a large proportion of all plasma protein. Low albumin (hypoalbuminaemia) may be caused by liver disease, nephrotic syndrome, burns, protein-losing enteropathy, malabsorption, malnutrition, late pregnancy, artefact, posture, genetic variations and malignancy. High albumin may be either caused by dehydration or other factors. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to give the secreted protein. Albumin is a globular, non-glycosylated, soluble, monomeric protein (65kD) which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in the ALB gene on chromosome 4 result in various anomalous proteins. Albumin is negatively charged. The glomerular basement membrane is also negatively charged; this prevents the filtration of albumin in the urine. In nephrotic syndrome, this property is lost, and there is more albumin loss in the urine. Nephrotic syndrome patients are given albumin to replace the lost albumin. Human serum albumin (HSA) is widely used to stabilize blood volume. HSA is typically obtained from donors. However, there is concern about the transmission of many diseases such as HIV & Hepatitis. Recombinant HSA (rHSA) can be produced in vitro (E. coli or mammalian cells) and therefore has no disease concerns. rHSA is identical to that in natural blood. \n\nRecombinant protein corresponding to Human HSA (rHSA) produced in mammalian cell line is a single, glycosylated, polypeptide chain containing 585aa (66.411kD). \n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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