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Alexa Fluor(R) 488 anti-mouse FOXP3 Flow Kit

Alexa Fluor(R) 488 anti-mouse FOXP3 Flow Kit

Cat no: 126411

Supplier: BioLegend
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FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 delta2) lacking exon 2. BioLegend's Alexa Fluor(R) 488 anti-mouse FOXP3 Flow Kit is designed and formulated specifically for immunofluorescence staining and flow cytometric analysis of mouse FOXP3+ Treg cells in a mixed lymphocyte population. This kit is composed of Alexa Fluor(R) 488 conjugated anti-mouse FOXP3 antibody and Alexa Fluor(R) 488 conjugated matching isotype control and the critical buffers. It is easy to use for identification of FOXP3+ Treg cells.
Catalogue number: 126411
Reactivities: Mouse, Rat
Hosts: Rat
Applications: Flow Cytometry, Immunocytochemistry
Size: 25 microg
Clone: MF-14
Gene: 20371
Dilutions: For immunofluorescent staining, the suggested use of this reagent is 5 microl per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application. Each lot of this FOXP3 antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis.* Alexa Fluor(R) 488 has a maximum emission of 519 nm when it is excited at 488 nm.** Alexa Fluor(R) is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: This kit is guaranteed for three months. Upon receipt, store at 4C and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Isotype: Rat IgG2b, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
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