AMICA, also known as junctional adhesion molecule-like protein (JAML), is a 60kD type I transmembrane glycoprotein in the CTX (cortical thymocyte marker in Xenopus) family of adhesion molecules. Like other CTX family proteins, AMICA has an extracellular domain containing one V- and one C-type Ig domain, a transmembrane domain and an intracellular tail that lacks a PDZ-binding motif. AMICA is expressed on neutrophils and participates in heterophilic adhesive interactions with CXADR at tight junctions of polarized epithelia. Two additional isoforms that differ either in the signal peptide or contain substitutions in residues 258 and 259, have been reported. Within their extracellular domains, human and mouse AMICA share approximately 55% aa sequence homology.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
ELISA (Detection): 0.1-0.4ug/ml can be used as a detection reagent in a human AMICA sandwich immunoassay in combination with the human AMICA capture reagent (A1372-31H4) and recombinant human AMICA as the standard. Shows <0.3% crossreactivity with recombinant mouse AMICA and recombinant human JAM-A, JAM-B and JAM-C.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.�
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