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AMPK beta1, phosphorylated (Ser181) (AMP-activated Protein Kinase)

AMPK beta1, phosphorylated (Ser181) (AMP-activated Protein Kinase)

Cat no: A1475-05D

Supplier: United States Biological
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The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising a (63kDa), b (38kDa) and g (38kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4). Phsopholyation on serine 24/25 and serine 182 has been linked to AMPK localization. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2,000-1:20,000 Immunohistochemistry: 1:100-1:250 Immunocytochemistry: 1:100-1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: A1475-05D
Reactivities: Human
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Serum
References: 1. Mitchelhill, K. I., et al., (1994) J. Biol. Chem. 269, 2361-2364 2. Woods, A.; et al..: J. Biol. Chem. 271: 10282- 10290, 1996. 3. Carling, D., et al., (1989) Eur. J. Biochem. 186, 129-136 4. Thornton, C.; et al.,. Biol. Chem. 273: 12443-12450, 1998.
Additional info: Recognizes human AMPD beta 1 when phosphorylated at Ser181.

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