AMPK beta1, phosphorylated (Ser181) (AMP-activated Protein Kinase)

The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising a (63kDa), b (38kDa) and g (38kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4). Phsopholyation on serine 24/25 and serine 182 has been linked to AMPK localization.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2,000-1:20,000 \nImmunohistochemistry: 1:100-1:250 \nImmunocytochemistry: 1:100-1:250 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

A1475-05D

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Serum

Isotype

IgG

References

1. Mitchelhill, K. I., et al., (1994) J. Biol. Chem. 269, 2361-2364 2. Woods, A.; et al..: J. Biol. Chem. 271: 10282- 10290, 1996. 3. Carling, D., et al., (1989) Eur. J. Biochem. 186, 129-136 4. Thornton, C.; et al.,. Biol. Chem. 273: 12443-12450, 1998.

Additional Info

Recognizes human AMPD beta 1 when phosphorylated at Ser181.