Angiogenin

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis. Angiogenin is a single polypeptide chain of 123aa (mol wt ~14kD) secreted by tumor cells. It is a potent inhibitor of neovascularization. It specifically binds to endothelial cells and elicits second messenger systems. It has ribonucleolytic activity and is 33% identical to pancreatic RNAse A. \n\nApplications:\nSuitable for use in Western Blot, ELISA and Neutralization. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1-5ug/ml using ECL.\nELISA: 0.5-2.0ug antibody and 10-50ng of control antigen/well.\nNeutralization: 100-200ug IgG neutralizes 50% activity of 1ug of Angiogenin. Neutralizes biological activity of recombinant human Angiogenin as measured by measurement of ribonucleolytic activity using tRNA. \nOptimal dilution determined by the researcher. \n\nRecommended Control:\nA2292-53C: Angiogenin, Recombinant, Human, Western Blot Control\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2292-53B

Size

50ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in 20mM Tris, pH 7.4, 0.1% BSA.

P Type

Pab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG

References

1. Kurachi, K., et al., Biochemistry 24: 5494-5499 (1985). 2. Fu, X., et al., Mol. Cell. Biol. 17: 1503-1512 (1997). 3. Aharya, K.R., et al., PNAS 91: 2915-2919 (1994). 4. Hu, G., et al., PNAS 91: 12,096 (1994). 5. Lee, F.S., et al., BBRC 161: 121 (1989). 6. Lee, F.S., BBRC 161: 121 (1989).

Additional Info

Recognizes human Angiogenin at ~14kD.