Angiotensin Converting Enzyme (ACE)

Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase anchored in the plasma membrane with a carboxy-terminal hydrophobic domain (1, 2). ACE has a critical role in cardiovascular function because it catalyses the conversion of angiotensin I to the physiologically active vasopressor octapeptide angiotensin II, which controls fluid-electrolyte balance and systemic blood pressure (3, 4). Inhibitors of ACE are a first line of therapy for hypertension, heart failure, myocardial infarction and diabetic nephropathy (4). ACE also plays a \ncritical role in the circulating or endocrine renin-angiotensin system (RAS) as well as the local regulation that exists in tissues such as the myocardium and skeletal muscle (5). \n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. \n\nRecommended Dilutions: \nWestern Blot: 1:500 -1000 \nImmunohistochemistry: 1:100-250 \nFlow Cytometry: 1:30 \nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

A2295-01B3

Size

100ul

Applications

FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Serum

Isotype

IgG

References

1. Ehlers MR, et al. Proc Natl Acad Sci U S A. 86(20): 7741-5, 1989 2. Sugimura K, et al. Biochem Biophys Res Commun. 247(2): 466-72, 1998 3. Rieder MJ, et al. Nat Genet. 22(1): 59-62, 1999. 4. Natesh R, et al. Nature. 421(6922): 551-4, 2003. 5. Natesh R, et al. Biochemistry. 43(27): 8718-24, 2004

Additional Info

Recognizes human ACE. Species Crossreactivity: mouse