ANGPTL6 induces angiogenensis and arteriogenesis through activation of the ERK1/2-eNOS pathway. Transgenic mice that persistently overexpress ANGPTL6 have increased systemic energy expenditure counteracting high-fat diet-induced obesity and related insulin resistance, while onset of obesity in ANGPTL6 null mice is attributed to decreased energy expenditure and insulin resistance. Cayman's Human ANGPTL6 EIA Kit is an immunometric (i.e., sandwich) assay that can be used to measure ANGPTL6 in human serum, plasma, or cell culture supernatants.