Ankyrin G (RFXANK, Regulatory Factor X)

Ankyrin G is found in several tissues including brain where, due to tissue-specific RNA processing, it is expressed as two different isoforms, localized to axon initial segments and nodes of Ranvier. Ankyrin G is also found at sites of epithelial cell- cell contact and in the intracellular membranes of some cells. Ankyrin G isoforms contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following a globular head domain. Ankyrin G is thought to participate in the maintenance and targeting of ion channels and cell adhesion molecules to the nodes of Ranvier and to axon initial segments.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nELISA (Native): 0.1-1.0ug/ml \nWestern Blot: 1-3ug/ml. For best results on rat brain, mouse brain or MDCK cell lysates, add 0.3M sucrose to incubation buffer. Detects a band between ~200-300kD.\nImmuno?uorescence (Frozen): 5-10ug/ml. Sensitive to fixation conditions. Recommend 1% paraformaldehde for perfusion and 4 hours of post fixation, after perfusion. For sciatic nerve, recommend 2% paraformaldehyde for 2 hours at 4C.\nImmunoprecipitation (Native): 5ug/ IP\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A2295-18

Size

100ug

Applications

ELISA, IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, Rabbit

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Mathis C, et al. Development 128:4881-4890, 2001. 2. Kordeli E, et al. J Biol Chem 270(5):2352-2359, 1995. 3. Kordeli E, et al. J Cell Sci 111:2197-2207, 1998. 4. Martin S, et al. J. Neurosci 21(20) : 8119-8128, 2001. 5. Malhotra JD, et al. J Biol chem. 277(29): 26681-26688, 2002

Additional Info

Recognizes human Ankyrin G. Does not crossreact with related ankyrin isoforms. Also recognizes all splice forms of Ankyrin G containing a spectrin-binding domain \nSpecies Crossreactivity: mouse, rabbit, rat and canine.