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anti-Ubiquitin antibody

Cat no: ABIN1043766

anti-Ubiquitin antibody

anti-Ubiquitin antibody: Ubiquitin (Ub) is a small, 76-residue, protein (8.5 kDa) found both as free monomer and covalently attached to itself and other proteins in eukaryotic cells. Free Ub is a very compact and stable molecule that is easily refolded after being denatured. It is therefore recommended that for detection of free Ub on Westerns, the Tris-Tricine SDS-PAGE is used and nitrocellulose filters are autoclaved after the transfer and before blocking and addition of anti-Ub antibodies. The C-terminus of ubiquitin forms an isopeptide bond with the e-amino group of a lysine side chain in a target protein. In this way proteins can be covalently modified by the addition of ubiquitin which may alter the target protein's function. Monoubiquitination generally targets proteins for internalization, endocytosis and lysosomal degradation, or modifies the surface charge of histones and affects chromatin compaction. Conjugation of ubiquitin (Ub) involves a three-step mechanism whereby specific enzymes (or enzyme complexes) activate and covalently link Ub to their substrates.

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SPECIFICATIONS

Catalog Number

ABIN1043766

Size

25 micro L

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Antigen

Ubiquitin

Gene Id

100716362,5740245,100135798,100125968

Isotype

IgG

Clonality

Polyclonal

Concentration

1.0 mg/mL

Entrez Gene Id

100716362,5740245,100135798,100125968

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