AP2M1 (CLAPM1, KIAA0109, AP-2 Complex Subunit mu, AP-2 mu Chain, Adapter-related Protein Complex 2 mu Subunit, Adaptin-mu2, Adaptor Protein Complex AP-2 Subunit mu, Clathrin Assembly Protein Complex 2 Medium Chain, Clathrin Coat Assembly Protein AP50, Cla

The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml\nImmunofluorescence: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Kidney Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146439

Size

100ug

Applications

ELISA, IF, IHC, WB

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgY

References

1. Robinson MS. Adaptable adaptors for coated vesicles. Trends Cell Biol. 2004; 14:167-74. 2. Aguilar RC, Ohno H, Roche KW, et al. Functional domain mapping of the clathrin-associated adaptor medium chains mu1 and mu J. Biol. Chem. 1997; 272:27160-6. 3. Liu Q, Feng Y, and Forgac M. Activity and in vitro reassembly of the coated vesicle (H+)-ATPase requires the 50-kDa subunit of the clathrin assembly complex AP-2. J. Biol. Chem. 1994; 269:31592-7. 4. Craig HM, Reddy TR, Riggs NL, et al. Interactions of HIV-1 Nef with the m subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting motif. Virology 271:9-17.

Additional Info

Recognizes human AP2M1. Species Crossreactivity: mouse and rat.