5'-G G G C C^C-3'
3'-C^C C G G G-5'
Concentration: 10u/ul
Source: Acetobacter pasteurianus
Buffer: 10mM Tris-HCl (pH 7.5), 10mM MgCl2 and 0.1mg/ml BSA. Incubate at 37 degrees C. (Incubation at 30 degrees C results in a 2-fold increase in activity.)
Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25 degrees C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods