APC (Adenomatous Polyposis coli)

Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling (1). APC regulates b-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration (2-3). Mutations cause the production of an APC protein that is abnormally short and nonfunctional. Defects in APC are a cause of familial aenomatous polyposis (FAP), Turcot syndrome, medulloblastoma (MDB), and other cancers. (4-6)\n\nApplications: \nSuitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting: 1:500-1,000\nImmunohistochemistry: 1:50-100\nImmunocytochemistry: 50-100\nImmunoprecipitation: 1:50\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

A2298-70F

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1.Senda T, Shimomura A, Iizuka-Kogo A. Anat Sci Int. 2005 Sep;80(3):121-31. 2.Narayan S, Roy D. Mol Cancer. 2003 Dec 12;2:41. 3.van Es JH, Giles RH, Clevers HC. Exp Cell Res. 2001 Mar 10;264(1):126-34. 4.Jarvinen HJ, Peltomaki P. Eur J Gastroenterol Hepatol. 2004 Jan;16(1):5-8. 5.Jaiswal AS, Balusu R, Narayan S. Front Biosci. 2005 May 1;10:1118-34. 6.Cohen MM Jr. Am J Med Genet A. 2003 Nov 1;122(4):303-14.

Additional Info

Recognizes human APC