APC, CT (Adenomatous Polyposis coli)

APC is a ubiquitous, tumor-suppressor protein that is mutated in most colon cancers, generally resulting in a gene product that is truncated in the carboxy terminal region. APC contains several binding domains. The region used as an immunogen overlaps the EB1, DLG (PDZ sequence) and PSD-93 domains. These domains bind microtubules, mitotic spindles and F-actin and are involved in cell cycle, division and migration. The immunogen does not include the beta-catenin-binding region, which antagonizes Wnt signaling. The human APC amino acid sequence used as immunogen is 83% and 86% identical to mouse and rat APC, respectively. \n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and ELISA. Optimal dilutions should be determined by each laboratory for each application.\n\nRecommended Dilutions:\nWestern Blot: 0.1-0.2ug/ml, the detection limit is~1ng/lane under non-reducing and reducing conditions. \nImmunohistochemistry: 5-15ug/ml\nELISA: 0.5-1ug/ml, the deterction limit is ~0.2ng/well.\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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