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Apolipoprotein A-IV (APOA4, MGC142154, MGC142156)

Apolipoprotein A-IV (APOA4, MGC142154, MGC142156)

Cat no: A2299-36B

Supplier: United States Biological
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Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. Applications: Suitable for use in ELISA and Western BlotOther applications not tested. Recommended Dilution: ELISA: 1:32000 Western Blot: 0.01-0.03ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A2299-36B
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Spaulding HL, Saijo F, Turnage RH, Alexander JS, Aw TY, Kalogeris TJ. Apolipoprotein A-IV attenuates oxidant-induced apoptosis in mitotic competent, undifferentiated cells by modulating intracellular glutathione redox balance. Am J Physiol Cell Physiol. 2006 Jan;290(1):C95-C103. Epub 2005 Aug 24.
Additional info: Recognizes APOA4 at ~45kD in Human Duodenum lysates (calculated MW of 45.4kD according to NP_000473.2).

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