Apolipoprotein L1 specifically recognises an epitope within the C-Terminal (CT) region of human Apolipoprotein L1 (APOL1), a secreted high density lipoprotein and member of the apolipoprotein L family, which interacts with apolipoprotein A-I (APOA1) in plasma. Two isoforms of APOL1 have been identified, which may play a role in lipid exchange and transport throughout the body, and possibly in the reverse transport of cholesterol from peripheral cells to the liver.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:8000
Western Blot: 1.0-3.0ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1904-28K: IgG, H&L (HRP) Pab RbxGt
I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.