ARF, p14 (p16beta) (BSA & Azide Free)

The INK4a-ARF locus encodes two unrelated proteins both of which function in tumor suppression. p14ARF arrests the cell cycle in a p53-dependent manner. p14ARF binds to mdm2 and promotes the rapid degradation of mdm2 protein required for mdm2 modification and concurrent p53 stabilization and accumulation. This interaction is mediated by the exon 1beta-encoded Nterminal domain of p14ARF and a C-terminal region of mdm2. Deletion of the ARF-INK4a locus simultaneously impairs both the INK4a-cyclin D/cdk4-RB and the ARF-mdm2-p53 pathways. \n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and \nImmunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions: \nWestern Blot: 5-10ug/ml for 2hrs at RT. \nImmunohistochemistry (formalin/paraffin): 5-10ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\nOptimal dilutions to be determined by the researcher.\n \nPositive Control: \nHeLa cells, cervical carcinoma or breast fibroadenoma. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A3330-04AX

Size

250ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See A3330-04A

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Zhang Y, et al. Cell, 92: 725

Additional Info

May show some additional bands with MW much higher than 14kD, however, the p14ARF area is relatively clean which permits an easy identification of the p14ARF band. For optimun results, immunoprecipitate p14ARF with Western blot. \nSpecies Crossreactivity: human.