ARG1 (Liver-type arginase, Type I arginase)

ARG1(arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component \nof the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes \n98% of the arginase activity in liver but is also present in red cells. Using a rat liver ARG1 cDNA clone \nto probe a human liver cDNA library, Haraguchi et al. (1987) isolated and characterized a cDNA \ncorresponding to the ARG1 gene. The ARG1 gene is mapped on 6q23.2 and the arginase gene \ncontains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was \nprecipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal \ntract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive \ndisorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have \nbeen found for this gene.\n\nUniProt Number:\nP07824\n\nGene ID:\nARG1\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144198

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Cardoso, M. L., Martins, E., Vasconcelos, R., Vilarinho, L., Rocha, J. Identification of a novel R21X \nmutation in the liver-type arginase gene (ARG1) in four Portuguese patients with argininemia. Hum. \nMutat. 14: 355-356, 1999.\n2. Grody, W. W., Dodson, A., Klein, D., Kern, R. M., Bassand, P., Cederbaum, S. D. Molecular genetic \nstudy of human arginase deficiency. (Abstract) Am. J. Hum. Genet. 45 (suppl.): A191 only, 1989.\n3. Haraguchi, Y., Takiguchi, M., Amaya, Y., Kawamoto, S., Matsuda, I., Mori, M. Molecular cloning and \nnucleotide sequence of cDNA for human liver arginase. Proc. Nat. Acad. Sci. 84: 412-415, 1987.

Additional Info

Recognizes mouse and rat ARG1. No crossreactivity with other proteins.