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ARHGEF5 (Rho Guanine Nucleotide Exchange Factor 5, TIM, Ephexin-3, Guanine Nucleotide Regulatory Protein TIM, Oncogene TIM, Transforming Immortalized Mammary Oncogene, p60 TIM)

ARHGEF5 (Rho Guanine Nucleotide Exchange Factor 5, TIM, Ephexin-3, Guanine Nucleotide Regulatory Protein TIM, Oncogene TIM, Transforming Immortalized Mammary Oncogene, p60 TIM)

Cat no: R1998-27E

Supplier: United States Biological
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Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 0.1-0.3ug/ml, observed in Human HeLa lysates on ~170kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: R1998-27E
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Debily MA, Camarca A, Ciullo M, Mayer C, El Marhomy S, Ba I, Jalil A, Anzisi A, Guardiola J, Piatier-Tonneau D. Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer. Hum Mol Genet. 2004 Feb 1;13(3):323-34. Epub 2003 Dec 8.
Additional info: Recognizes human ARHGEF5.

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