ATF2 (Phospho-Thr69,71) Transcription Factor Assay Kit

ATF2 is a sequence-specific DNA-binding protein belonging to the bZIP family of transcription factors that bind with high affinity to the octameric CRE. ATF2 mediates both transcription and DNA damage control through its phosphorylation/activation in response to inflammatory cytokines, UV irradiation, alkylating compounds, and other cellular stressors.

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SPECIFICATIONS

Catalog Number

600130

Form

96 Well

P Type

Assay Kits|Transcription Factors|Nuclear Receptors|Intracellular Signaling

Weight

0

Storage Temp

-80

Shipping Temp

-80

Additional Info

ATF2 (Activating Transcription Factor 2) is a sequence-specific DNA-binding protein belonging to the bZIP family of transcription factors that bind with high affinity to the octameric cyclic AMP-responsive element (CRE) in the promoter region of target genes. ATF2 mediates both transcription and DNA damage control through its phosphorylation/activation in response to inflammatory cytokines, UV irradiation, alkylating compounds, and other cellular stressors. The activated Phospho-Thr69,71 species of ATF2 (pThr69,71 ATF2; phosphorylated at both Thr69 and Thr71) is specifically mediated by JNK/p38. phospho-Thr69,71 ATF2 can form homodimers or selective heterodimers with other members of the ATF/CREB family including Fos, Fra2, c-Jun, CREB and ATF1, yielding a diverse array of DNA-binding complexes which contribute to regulatory specificity among bZIP family proteins. Recent studies implicate the ATF/CREB family of transcription factors in key cellular processes including cell cycle control, drug resistance, apoptosis, tumorigenesis, and insulin biosynthesis. Cayman's ATF2 (Phospho-Thr69,71) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the cAMP-response element (CRE) is immobilized onto the wells of a 96-well plate. ATF2 contained in a nuclear extract, binds specifically to the CRE. The activated ATF2 transcription factor complex is detected by addition of a specific primary antibody directed against the phospho-Thr69,71 epitope on ATF2. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.