ATP6V1A, ID (ATP6V1A, ATP6A1, ATP6V1A1, VPP2, V-type proton ATPase catalytic subunit A, V-ATPase 69kD subunit, Vacuolar ATPase isoform VA68, Vacuolar proton pump subunit alpha) (AP)

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nImmunohistochemistry: 1:50-100\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

032296-AP

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Martins-de-Souza, D., et al. J Psychiatr Res 43(11):978-986(2009)\nMartins-de-Souza, D., et al. BMC Psychiatry 9, 17 (2009) \nLu, M., et al. J. Biol. Chem. 282(34):24495-24503(2007)\nAhmed, M., et al. J. Proteome Res. 4(3):931-940(2005)\nMorel, N. Biol. Cell 95(7):453-457(2003)

Additional Info

Human

Alternative Names

ATP6V1A, ATP6A1, ATP6V1A1, VPP2, V-type proton ATPase catalytic subunit A, V-ATPase 69kD subunit, Vacuolar ATPase isoform VA68, Vacuolar proton pump subunit alpha