B Cell Linker Protein (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65)

BLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70kD in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B0397-01D

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Fu C et al. BLNK: a central linker protein in B cell activation. Immunity 9:93-103 (1998). <PUBMED:9697839>

Additional Info

Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse due to sequence homology.

Alternative Names

B Cell Adapter Containing SH2 Domain Protein, B Cell Adapter Containing Src Homology 2 Domain Protein, Bca, Cytoplasmic Adapter Protein, Ly 57, Lymphocyte antigen 57, Lyw 57, Lyw57, SLP 65, Src Homology 2 Domain Containing Leukocyte Protein of 65kD