B7H4 (B7-H4, B7S1, B7x, BC032925, Immune Costimulatory Protein B7H4, MGC41287, PRO1291, T Cell Costimulatory Molecule B7x, V Set Domain-containing T Cell Activation Inhibitor 1, VCTN1) (PE)

B7H4, also known as B7x, is a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7H4 deficient mice are only minimally affected; suggesting B7H4 is important in the fine tuning of the T-cell mediated immune response. B7H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 could be a useful prognostic marker in Renal Cell Carcinoma (RCC).\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: Neat. 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nRecommended Negative Control:\nI1904-79D: IgG1 Murine Negative Control (PE)\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Reconstitute with sterile ddH2O. Aliquot and store at 4 degrees C. DO NOT FREEZE. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

B0000-35B5

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. van de Ven, R., et al., Blood 118: 2502-2510 (2011).

Additional Info

Recognizes human B7H4.