BACE1 (Beta-site Amyloid Precursor Protein Cleaving Enzyme 1, Beta-site APP Cleaving Enzyme 1, BACE, Aspartyl Protease 2, Asp 2, ASP2, Beta-secretase 1, FLJ90568, HSPC104, KIAA1149, Memapsin-2, Membrane-associated Aspartic Protease 2)

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Four transcript variants encoding different isoforms have been described for this gene. Bace 1 is responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman brain, Alzheimers\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

B0002-88K

Size

50ug

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Bioorg Med Chem Lett 2006 Oct. : Design of potent inhibitors of human beta-secretase. Part 2. Freskos JN, Fobian YM, Benson TE, Moon JB, Bienkowski MJ, Brown DL, Emmons TL, Heintz R, Laborde A, McDonald JJ, Mischke BV, Molyneaux JM, Mullins PB, Bryan Prince D, Paddock DJ, Tomasselli AG, and Winterrowd G. 2. Neurodegener Dis 2006 . 3:270-4 Amyloid Precursor Protein and BACE Function as Oligomers. Multhaup G. 3. Neurodegener Dis 2006 . 3:262-9 Ectodomain shedding of the amyloid precursor protein: cellular control mechanisms and novel modifiers. Lichtenthaler SF. 4. Neurodegener Dis 2006 . 3:247-54 Control of Amyloid-beta-Peptide Generation by Subcellular Trafficking of the beta-Amyloid Precursor Protein and beta-Secretase. Walter J. 5. Neurodegener Dis 2006 . 3:218-26 Subcellular trafficking of the amyloid precursor protein gene family and its pathogenic role in Alzheimer's disease. Kins S, Lauther N, Szodorai A, and Beyreuther K.

Additional Info

Recognizes human BACE1/ASP2. Species Sequence Homology: rat.

Alternative Names

Beta-site APP-cleaving Enzyme 1, EC=3.4.23.46