Western Blot ECL Kit with Enhancer, Anti-Chicken, BioAssay(TM)

Ultra-sensitive ECL Western Blot System that detects attogram (10e-18 g) or yoctomole (10e-24M) protein levels. The Enhancer utilizes a proprietary technology that increases antigen and antibody affinity and accessibility which can work with different antigens and antibodies, such as structural proteins (Actin), enzymes (GAPDH) or membrane proteins (EGFR), increasing Western blot signals dramatically.\n\nKit Components:\nComponents are prepared in accordance with protocols suitable for use in ultra-sensitive chemiluminescent analysis of Western Blot (ECL) and provide a complete system for Western Blot analysis, including:\n1. Enhancer, to boost western blot signals\n2. Protein Interaction System\n3. Chemiluminescent Detection System\n4. Proprietary Blocking Agent\n\nFour customized formats: \nHRP-conjugated anti Mouse, anti-Rabbit, anti-Chicken, anti-Goat IgG\n\nTwo convenient and affordable sizes:\n15 Mini Blots (1200cm2) and 30 Mini Blots (2500cm2).\n\nQuality Control:\nDuplicated Western blots containing 10fg and 10pg of antigen are detected with anti-GAPDH antibody. Antigen at 10fg levels was detected only on the blot treated with Enhancer, with similar intensity as antigen at 10pg level on the untreated blot.\n\nOne ng of purified Rabbit muscle GAPDH was serially diluted to 1 attogram and electrophoresis was performed. The gel was transferred to a PVDF membrane, and then cut in the middle. The right section was treated with Enhancer, and the left was untreated. Immunoblotting was done using a primary antibody and HRP-conjugated rabbit anti-mouse antibody, and chemiluminescent substrates were applied. A film exposed for two minutes is shown above. 10-e11g of antigen without treatment and 10e-14g of GAPDH with treatment gave similar signal strengths, indicating a 1000-fold enhancement*.\n* Fold enhancement varied from a few to a 1000-fold depending on the characteristics of antibodies and antigen used.\n\nNote:\nComponents in this kit are free of precipitate. When testing by visible spectrophotometry, the color of the solution is comparable to a control Lot. Results from each lot are comparable to those obtained with control lot of the solution.\n\nShelf Life: \n1 year from the date of receipt under proper storage conditions

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts