Bad, phosphorylated (Ser112) (Bcl2 Antagonist of Cell Death)

Bad is a proapoptotic member of the Bcl-2 family that can displace Bax from binding to Bcl-2 and Bcl-XL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-2 and Bcl-XL. Akt has been shown to promote cell survival via its ability to phosphorylate Bad at Ser136. Ser112 has been shown to be the substrate in vivo and in vitro of p90RSK and mitochondria-anchored PKA. Phosphorylation of Ser155 by PKA plays a critical role in blocking the dimerization of Bad to Bcl-XL because of its position in the BH3 domain.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\n\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B0003-57

Size

100ul

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Yang, E. et al. (1995) Cell 80, 285

Additional Info

Detects endogenous levels of total Bad only when phosphorylated at Ser112. Does not detect Bad phosphorylated at other sites. Nor does it detect related family members. Species Crossreactivity: Human, mouse, monkey and rat. Other species have not been tested.