Bcl 2a (B Cell Leukemia 2a)

Expression of bcl-2a oncoprotein has been shown to inhibit the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2a protein, whereas the normal or hyperplastic germinal centers are negative.\n\nBcl-2 belongs to a family of genetically related proteins that appear to regulate apoptosis or programmed cell death. Bcl-2 is encoded by a proto-oncogene (bcl-2) that is the mammalian homolog of ced-9, the C. elegans death suppressor gene. Overexpression of Bcl-2 appears to suppress apoptosis in a number of mammalian experimental systems. Recent evidence indicates that Bcl-2 binds to other family members and R-ras and influences antioxidant pathways in the cell. Bcl-2 is a 25kDa protein that has extensive amino acid homology with Bax. It forms homodimers or heterodimers with Bax and the apoptotic activity depends on the balance of both molecules. The t(14;18) translocation, which is a hallmark of human follicular lymphoma, generally involves the immunoglobulin heavy chain gene on chromosome 14 and the bcl-2 gene on chromosome 18. This translocation results in overexpression of the bcl-2 gene product (Bcl-2), promoting cell survival by blocking programmed cell death induced by different apoptotic stimuli. \n\nApplications: ELISA (For coating, Ab can be ordered without BSA), Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (frozen & formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.\n\nRecommended Dilution:\nWestern Blotting: 1ug/ml\nImmunohistochemistry: 1-2ug/ml

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SPECIFICATIONS

Catalog Number

B0807-07B

Size

500ul

Applications

ELISA, FC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, NHP, Prc

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.\nAlso available without BSA and azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.1. Alderson LM; Castleberg RL; Harsh GR 4th; Louis DN; Henson JW. Human gliomas with wild-type p53 express bcl-2. Cancer Research, 1995 Mar 1, 55(5):999-1001.2. Ashton-Key M; Biddolph SC; Stein H; Gatter KC; Mason DY. Heterogeneity of bcl-2 expression in MALT lymphoma. Histopathology, 1995 Jan, 26(1):75-8.\n3. Bargou RC; Daniel PT; Mapara MY; Bommert K; Wagener C; Kallinich B; Royer HD; Dorken B. Expression of the bcl-2 gene family in normal and malignant breast tissue: low bax-alpha expression in tumor cells correlates with resistance towards apoptosis. International Journal of Cancer, 1995 Mar 16, 60(6):854-9.\n4. Bensi L; Longo R; Vecchi A; Messora C; Garagnani L; Bernardi S; Tamassia MG; Sacchi S. Bcl-2 oncoprotein expression in acute myeloid leukemia. Haematologica, 1995, 80(2):98-102.\n5. Bosari S; Moneghini L; Graziani D; Lee AK; Murray JJ; Coggi G; Viale G. bcl-2 oncoprotein in colorectal hyperplastic polyps, adenomas, and adenocarcinomas. Human Pathology, 1995 May, 26(5):534-40.\n6. Ceccarelli C; Santini D; Chieco P; Taffurelli M; Marrano D; Mancini AM. Multiple expression patterns of biopathological markers in primary invasive breast carcinoma: a useful tool for elucidating its biological behaviour. Annals of Oncology, 1995 Mar, 6(3):275-82.\n7. Cerroni L; Soyer HP; Kerl H. bcl-2 protein expression in cutaneous malignant melanoma and benign melanocytic nevi. American Journal of Dermatopathology, 1995 Feb, 17(1):7-11.\n8. Chetty R; O

Additional Info

Recognizes human Bcl-2a at ~25kD. Species Reactivity: Human, monkey and porcine. Does not react with mouse or rat.