Home  >  Products  >  BOB1 (OCAB, POU2AF1, BOB1, OBF1, OCAB, OBF-1, POU domain, class 2, associating factor 1)
BOB1 (OCAB, POU2AF1, BOB1, OBF1, OCAB, OBF-1, POU domain, class 2, associating factor 1)

BOB1 (OCAB, POU2AF1, BOB1, OBF1, OCAB, OBF-1, POU domain, class 2, associating factor 1)

Cat no: B2525-02

Supplier: United States Biological
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OCA-B is a lymphoid-specific transcriptional coactivator which interacts with the transcription factors Oct-1 and Oct-2 (1). It has been shown to be critical for development of a normal immune response, where it mediates octamer-dependent transcriptional activity in B lymphocytes. It is also critically involved in the formation of germinal centers in secondary lymphoid organs. OCA-B levels have been observed to be massively upregulated in germinal center B cells, as compared with resting B cells. SIAH1 and SIAH2, known regulators of protein stability, have been identified as two related OCA-B interacting proteins. SIAH1 has been suggested as part of a novel regulatory loop controlling the level of OCA-B protein in B cells. B-cell receptor cross-linking results in a degradation of OCA-B, which further results in a reduction of transcriptional activation of OCA-B-dependent reporters (2, 3). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5,000-30,000 Immunohistochemistry: 1:100-250 Immunocytochemistry: 1:100-250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: B2525-02
Reactivities: Human
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
Alternative names: B-cell-specific coactivator OBF-1, OCT-binding factor 1, BOB-1, OCA-B
References: 1. Bartholdy B, et al. Proc Natl Acad Sci U S A. 103(31):11665-70, 2006 2. Tiedt R, et al. EMBO J. 20(15):4143-52, 2001 3. Boehm J, et al. EMBO J. 20(15):4153-62, 2001
Additional info: Recognizes human OCA-B.

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