Brain Derived Neurotrophic Factor, pro (proBDNF)

BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease.\n\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-5ug/ml. Detects a 35kD band corresponding to the molecular weight of proBDNF.\nImmunohistochemistry: 1-5ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

B2700-02K

Size

20ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Reconstitute in 20ul of sterile water.

P Type

Pab

Purity

Purified by affinity chromatography.

References

1. A Acheson et al (1995) Nat. 74: 450-3 ; 2. Q Yan et al (1994) J. Neurosci. 14(9): 5281-91 ; 3. XF Zhou et al (1996) Neurosci. 74: 945-53 ; 4. XF Zhou, et al (1998) Exp. Neurol. 149: 237-42 ; 5. B Mellstrom et al (2004) Crit Rev Neurobiol 16, 43-9 ; 6. I Tapia-Arancibia et al (2004) Front Neuroendocrinol 25, 77-107 ; 7. S Pezet, et al (2002) Brain Res Brain Res Rev 40, 240-9 ; 8. Barde Y. A. et al (1989) EMBO J. 1: 549 ; 9. Conner J et al. (1997) J. Neurosci. 17: 2295

Additional Info

Recognizes human proBDNF. Species Crossreactivity: mouse and rat. Expected to recognize other mammalian proBDNF.

Alternative Names

Abrineurin