logo
logo
BRG1 bromodomain TR-FRET Assay Kit

Cat no: 600720

BRG1 bromodomain TR-FRET Assay Kit

The SWI/SNF family of ATP-dependent chromatin remodeling enzymes catalyzes structural changes that allow proteins to access the nucleosomal DNA, alter the position of the nucleosomes on the DNA, or eject the histone octamer from the template. Mammalian SWI/SNF remodelers contain either BRM or BRG1 as their catalytic subunit. These subunits also contain a C-terminal bromodomain that binds to acetylated residues on histone tails. Stereotypical inhibitors of BET family bromodomains (JQ-1 and I-BET) do not inhibit the interaction of BRG1 with peptide binding partners (IC50 >100microM). Several subunits of the SWI/SNF complex exhibit tumor suppressor activity and BRG1 is mutated or expression is absent in a significant number of non-small-cell lung cancer samples. Cayman's BRG1 bromodomain TR-FRET Assay Kit is a homogeneous, TR-FRET assay method amenable to rapid characterization of inhibitors of bromodomain/acetylated peptide interaction in a high throughput format. This screening assay is robust, displaying a Z' >0.7.

Prices direct from Cayman Chemical Company

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

600720

Form

384 Well

P Type

Assay Kits|Acetyl Reader

Weight

0

Storage Temp

-80

Shipping Temp

-80

Additional Info

The SWI/SNF family of ATP-dependent chromatin remodeling enzymes catalyzes structural changes that allow proteins to access the nucleosomal DNA, alter the position of the nucleosomes on the DNA, or eject the histone octamer from the template. Mammalian SWI/SNF remodelers contain either BRM or BRG1 as their catalytic subunit. These subunits also contain a C-terminal bromodomain that binds to acetylated residues on histone tails. Stereotypical inhibitors of BET family bromodomains (JQ-1 and I-BET) do not inhibit the interaction of BRG1 with peptide binding partners (IC50 >100microM). Several subunits of the SWI/SNF complex exhibit tumor suppressor activity and BRG1 is mutated or expression is absent in a significant number of non-small-cell lung cancer samples. Cayman's BRG1 bromodomain TR-FRET Assay Kit is a homogeneous, TR-FRET assay method amenable to rapid characterization of inhibitors of bromodomain/acetylated peptide interaction in a high throughput format. This screening assay is robust, displaying a Z' >0.7.

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

Genovis introduces GlycINATOR™• Works on most IgGs• Effective on high-mannose and bisected N-linked Fc-glycans • Rapid – 30-minute...

Use promo code EASY3 to receive 13% off and FREE shipping!Experience a new dimension of electronic pipetting with the NEW Easypet 3 pipette controller. The...

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...