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Bromodeoxyuridine (BrdU)

Cat no: B2850-09

Bromodeoxyuridine (BrdU)

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.\n\nApplications: \nSuitable for use in Immunohistochemistry and Flow cytometry. Other applications have not been tested. \n\nRecommended Dilutions: \nImmunohistochemistry: 1:1000-1:1250 on deparaffinized sections of kidney, duodenum, and liver tissue\nFlow Cytometry: 1:5000; Use 100ul of 10e6cells.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B2850-09

Size

100ug

Applications

FC, IHC

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A Sepharose chromatography.

Isotype

IgG1

References

1.Chang W.Y., et al., Biotechniques (2000) 29:934

Additional Info

Recognizes BrdU (Bromodeoxyuridine). Does not cross-react with fluodeoxyuridine, thymidine or uridine.

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