Nac1 is a transcriptional repressor with a BTB domain. In humans the protein is upregulated in a number of carcinomas and is localized in discrete nuclear bodies that correlate with tumor recurrence.
Positive Control: Human Bone Marrow lysate
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:2000.
Western Blot: 0.5-1.5ug/ml. ~55kD band observed in Human Bone Marrow lysates (calculated MW of 57.3kD according to NP_443108.1).
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.