BUBR1, NT (Mitotic Checkpoint Serine/Threonine-protein Kinase BUB1 beta, MAD3/BUB1-related Protein Kinase, hBUBR1, Mitotic Checkpoint Kinase MAD3L, Protein SSK1, BUB1B, MAD3L, SSK1) (Biotin)

BUB1B, a member of the Ser/Thr protein kinase family, is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. It can interact with BUB3, CENP-F, CENP-E and mitosin, and can be localized to nuclear kinetochores. This protein is highly expressed in thymus and spleen. The CD1 domain directs kinetochore localization and binding to BUB3. The protein possesses a cyclin destruction box sequence, which targets protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase. Defects in BUB1B are associated with tumor formation.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:50-1:200\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

B2790-40C-Biotin

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.Cahill, D.P., et al., Genomics 58(2):181-187 (1999). 2.Davenport, J.W., et al., Genomics 55(1):113-117 (1999). 3.Chan, G.K., et al., J. Cell Biol. 143(1):49-63 (1998). 4.Cahill, D.P., et al., Nature 392(6673):300-303 (1998). 5.Taylor, S.S., et al., J. Cell Biol. 142(1):1-11 (1998).

Additional Info

Recognizes human BUB1B.