UBTF, CT (UBTF, UBF, UBF1, Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1) (APC)

Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

043556-APC

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Hisaoka, M., et al. Mol. Cell. Biol. 30(20):4952-4964(2010)\nLiu, Z., et al. Proteins 77(2):432-447(2009)\nLin, C.I., et al. Biochem. Biophys. Res. Commun. 386(2):396-401(2009)\nGrob, A., et al. J. Cell. Sci. 122 (PT 4), 489-498 (2009) :\nHe, J., et al. Folia Biol. (Praha) 54(6):202-206(2008)

Additional Info

Human

Alternative Names

UBTF, UBF, UBF1, Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1